Atypical power myeloid leukemia (aCML) is a BCR-ABL1-negative clonal dysfunction, which belongs to the myelodysplastic/myeloproliferative group. This illness is characterised by recurrent somatic mutations in SETBP1, ASXL1 and ETNK1 genes, in addition to excessive genetic heterogeneity, thus posing an amazing therapeutic problem. To offer a complete genomic characterization of aCML we utilized a high-throughput sequencing technique to 43 aCML samples, together with each whole-exome and RNA-sequencing information. Our dataset identifies ASXL1, SETBP1, and ETNK1 as probably the most often mutated genes with a complete of 43.2%, 29.7 and 16.2%, respectively.
We characterised the clonal structure of seven aCML sufferers via colony assays and focused resequencing. The outcomes point out that ETNK1 variants happen early within the clonal evolution historical past of aCML, whereas SETBP1 mutations typically signify a late occasion. The presence of actionable mutations conferred each ex vivo and in vivo sensitivity to particular inhibitors with proof of sturdy in vitro synergism in case of a number of concentrating on. In a single affected person, a scientific response was obtained. Stratification primarily based on RNA-sequencing recognized two completely different populations when it comes to total survival, and differential gene expression evaluation recognized 38 considerably overexpressed genes within the worse consequence group. Three genes appropriately categorised sufferers for total survival.
Information Sanitization to Cut back Personal Info Leakage from Useful Genomics
The technology of useful genomics datasets is surging, as a result of they supply perception into gene regulation and organismal phenotypes (e.g., genes upregulated in most cancers). The intent behind useful genomics experiments is just not essentially to review genetic variants, but they pose privateness issues attributable to their use of next-generation sequencing. Furthermore, there’s a nice incentive to broadly share uncooked reads for higher statistical energy and normal analysis reproducibility.
Thus, we’d like new modes of sharing past conventional controlled-access fashions. Right here, we develop a data-sanitization process permitting uncooked useful genomics reads to be shared whereas minimizing privateness leakage, enabling principled privacy-utility trade-offs. Our protocol works with conventional Illumina-based assays and newer applied sciences corresponding to 10x single-cell RNA sequencing. It entails quantifying the privateness leakage in reads by statistically linking examine members to recognized people. We carried out these linkages utilizing information from extremely correct reference genomes and extra reasonable environmental samples.
The inheritance of variants that result in coding modifications in, or the mis-expression of, genes important to pancreatic beta cell operate can result in alterations in insulin secretion and enhance the chance of each sort 1 and kind 2 diabetes. Just lately developed clustered repeatedly interspaced brief palindromic repeats (CRISPR/Cas9) gene modifying instruments present a robust technique of understanding the affect of recognized variants on cell operate, development, and survival and would possibly finally present a way, almost definitely after the transplantation of genetically “corrected” cells, of treating the illness. Right here, we assessment a number of the disease-associated genes and variants whose roles have been probed to this point. Subsequent, we survey latest thrilling developments in CRISPR/Cas9 expertise and their attainable exploitation for β cell useful genomics. Lastly, we’ll present a perspective as to how CRISPR/Cas9 expertise might discover scientific software in sufferers with diabetes.
A zebrafish useful genomics mannequin to research the position of human A20 variants in vivo
Germline loss-of-function variation in TNFAIP3, encoding A20, has been implicated in all kinds of autoinflammatory and autoimmune circumstances, with acquired somatic missense mutations linked to most cancers development. Moreover, human sequence information reveals that the A20 locus comprises ~ 400 non-synonymous coding variants, that are largely uncharacterised. The rising variety of A20 coding variants with unknown operate, however potential scientific affect, poses a problem to conventional mouse-based approaches. Right here we report the event of a novel useful genomics strategy that makes use of a brand new A20-deficient zebrafish (Danio rerio) mannequin to research the affect of TNFAIP3 genetic variants in vivo.
A20-deficient zebrafish are hyper-responsive to microbial immune activation and exhibit spontaneous early lethality. Ectopic addition of human A20 rescued A20-null zebrafish from lethality, whereas missense mutations at two conserved A20 residues, S381A and C243Y, reversed this protecting impact. Ser381 represents a phosphorylation website vital for enhancing A20 exercise that’s abrogated by its mutation to alanine, or by a causal C243Y mutation that triggers human autoimmune illness. These information reveal an evolutionarily conserved position for TNFAIP3 in limiting irritation within the vertebrate linage and present how this operate is managed by phosphorylation. Additionally they reveal how a zebrafish useful genomics pipeline could be utilized to research the in vivo significance of medically related human TNFAIP3 gene variants.
Pancreatic ductal adenocarcinoma (PDA) is a lethal most cancers characterised by complicated metabolic diversifications that promote survival in a severely hypoxic and nutrient-limited tumor microenvironment (TME). Modeling microenvironmental influences in cell tradition has been difficult, and technical limitations have hampered the great examine of tumor-specific metabolism in vivo.
 Chicken Gizzard Total RNA* |
|
CR-105 |
Zyagen |
0.05mg |
EUR 160 |
 Chicken Gizzard Total Protein |
|
CT-105 |
Zyagen |
0.5mg |
EUR 153 |
, Chicken gizzard) Myosin S1 Fragment (smooth), Chicken gizzard |
|
CS-MYS05 |
Cytoskeleton |
1 x 250 µg |
EUR 383.76 |
) Actin protein (chicken gizzard muscle, >99% pure) |
|
AS99-A |
Cytoskeleton |
1 x 1 mg |
EUR 138.32 |
Actin protein (chicken gizzard muscle, >99% pure) |
|
AS99-B |
Cytoskeleton |
5 x 1 mg |
EUR 579.28 |
) Purified smooth muscle actin Protein (chicken gizzard) |
|
ACTB19-N-100 |
Alpha Diagnostics |
100 ug |
EUR 226.8 |
Purified smooth muscle actin Protein (chicken gizzard) |
|
ACTB19-N-1000 |
Alpha Diagnostics |
1 mg |
EUR 634.8 |
 control for Western) Purified smooth muscle actin Protein (chicken gizzard) control for Western |
|
ACTB19-C |
Alpha Diagnostics |
100 ul |
EUR 343.2 |
 IgG, purified) Monoclonal Anti-smooth muscle (gamma, alpha, chicken gizzard) IgG, purified |
|
ACTB19-M |
Alpha Diagnostics |
100 ug |
EUR 578.4 |
 43018-2 CK GIZZARD 25 EA* |
|
43018-2 |
Pel-Freez |
25 EA |
EUR 333 |
) Filamin protein (Chicken) |
|
30R-AF016 |
Fitzgerald |
250 ug |
EUR 431 |
|
|
|
Description: Highly purified Filamin protein |
Filamin protein (Chicken) |
|
MBS537578-025mg |
MyBiosource |
0.25mg |
EUR 575 |
Filamin protein (Chicken) |
|
MBS537578-5x025mg |
MyBiosource |
5x0.25mg |
EUR 2425 |
 Antibody Pair Kit (with Standard)) Human Filamin B Beta (FLNb) Antibody Pair Kit (with Standard) |
|
MBS2103238-10x96Tests |
MyBiosource |
10x96Tests |
EUR 1480 |
Human Filamin B Beta (FLNb) Antibody Pair Kit (with Standard) |
|
MBS2103238-10x96TestsMBS2090685AbPairsSupportPack110x96Tests |
MyBiosource |
10x96Tests+MBS2090685(AbPairsSupportPack1,10x96Tests) |
EUR 1600 |
Human Filamin B Beta (FLNb) Antibody Pair Kit (with Standard) |
|
MBS2103238-5x96Tests |
MyBiosource |
5x96Tests |
EUR 935 |
Human Filamin B Beta (FLNb) Antibody Pair Kit (with Standard) |
|
MBS2103238-5x96TestsMBS2090685AbPairsSupportPack15x96Tests |
MyBiosource |
5x96Tests+MBS2090685(AbPairsSupportPack1,5x96Tests) |
EUR 1010 |
 Antibody Pair Kit (with Standard)) Human Filamin A Alpha (FLNa) Antibody Pair Kit (with Standard) |
|
MBS2090267-10x96Tests |
MyBiosource |
10x96Tests |
EUR 1750 |
Human Filamin A Alpha (FLNa) Antibody Pair Kit (with Standard) |
|
MBS2090267-10x96TestsMBS2090685AbPairsSupportPack110x96Tests |
MyBiosource |
10x96Tests+MBS2090685(AbPairsSupportPack1,10x96Tests) |
EUR 1875 |
Human Filamin A Alpha (FLNa) Antibody Pair Kit (with Standard) |
|
MBS2090267-5x96Tests |
MyBiosource |
5x96Tests |
EUR 1100 |
Human Filamin A Alpha (FLNa) Antibody Pair Kit (with Standard) |
|
MBS2090267-5x96TestsMBS2090685AbPairsSupportPack15x96Tests |
MyBiosource |
5x96Tests+MBS2090685(AbPairsSupportPack1,5x96Tests) |
EUR 1165 |
 Antibody Pair Kit (with Standard)) Human Filamin C Gamma (FLNC) Antibody Pair Kit (with Standard) |
|
MBS2103239-10x96Tests |
MyBiosource |
10x96Tests |
EUR 1480 |
Human Filamin C Gamma (FLNC) Antibody Pair Kit (with Standard) |
|
MBS2103239-10x96TestsMBS2090685AbPairsSupportPack110x96Tests |
MyBiosource |
10x96Tests+MBS2090685(AbPairsSupportPack1,10x96Tests) |
EUR 1600 |
Human Filamin C Gamma (FLNC) Antibody Pair Kit (with Standard) |
|
MBS2103239-5x96Tests |
MyBiosource |
5x96Tests |
EUR 935 |
Human Filamin C Gamma (FLNC) Antibody Pair Kit (with Standard) |
|
MBS2103239-5x96TestsMBS2090685AbPairsSupportPack15x96Tests |
MyBiosource |
5x96Tests+MBS2090685(AbPairsSupportPack1,5x96Tests) |
EUR 1010 |
 Recombinant Chicken Filamin, partial |
|
MBS7137721-002mgBaculovirus |
MyBiosource |
0.02mg(Baculovirus) |
EUR 1145 |
Recombinant Chicken Filamin, partial |
|
MBS7137721-002mgEColi |
MyBiosource |
0.02mg(E-Coli) |
EUR 780 |
Recombinant Chicken Filamin, partial |
|
MBS7137721-002mgYeast |
MyBiosource |
0.02mg(Yeast) |
EUR 935 |
Recombinant Chicken Filamin, partial |
|
MBS7137721-01mgEColi |
MyBiosource |
0.1mg(E-Coli) |
EUR 935 |
Recombinant Chicken Filamin, partial |
|
MBS7137721-01mgYeast |
MyBiosource |
0.1mg(Yeast) |
EUR 1095 |
 Chicken Filamin B, β ELISA kit |
|
E01A91700 |
BlueGene |
96T |
EUR 700 |
|
Description: ELISA |
 Chicken Filamin B, Beta ELISA Kit |
|
MBS030883-INQUIRE |
MyBiosource |
INQUIRE |
Ask for price |
 Chicken Filamin B Beta ELISA Kit |
|
MBS078874-INQUIRE |
MyBiosource |
INQUIRE |
Ask for price |
Chicken Filamin B, Beta ELISA Kit |
|
MBS740299-10x96StripWells |
MyBiosource |
10x96-Strip-Wells |
EUR 5685 |
Chicken Filamin B, Beta ELISA Kit |
|
MBS740299-48StripWells |
MyBiosource |
48-Strip-Wells |
EUR 485 |
Chicken Filamin B, Beta ELISA Kit |
|
MBS740299-5x96StripWells |
MyBiosource |
5x96-Strip-Wells |
EUR 3020 |
Chicken Filamin B, Beta ELISA Kit |
|
MBS740299-96StripWells |
MyBiosource |
96-Strip-Wells |
EUR 690 |
 Chicken Filamin A, Alpha ELISA kit |
|
E01A91699 |
BlueGene |
96T |
EUR 700 |
|
Description: ELISA |
 Chicken Filamin C, Gamma ELISA kit |
|
E01A91701 |
BlueGene |
96T |
EUR 700 |
|
Description: ELISA |
 Chicken Filamin C Gamma ELISA Kit |
|
MBS091212-INQUIRE |
MyBiosource |
INQUIRE |
Ask for price |
 Chicken Filamin A Alpha ELISA Kit |
|
MBS061560-INQUIRE |
MyBiosource |
INQUIRE |
Ask for price |
 Chicken Filamin A, Alpha ELISA Kit |
|
MBS036883-INQUIRE |
MyBiosource |
INQUIRE |
Ask for price |
 Chicken Filamin C, Gamma ELISA Kit |
|
MBS033659-INQUIRE |
MyBiosource |
INQUIRE |
Ask for price |
Chicken Filamin C, Gamma ELISA Kit |
|
MBS735191-10x96StripWells |
MyBiosource |
10x96-Strip-Wells |
EUR 5685 |
Chicken Filamin C, Gamma ELISA Kit |
|
MBS735191-48StripWells |
MyBiosource |
48-Strip-Wells |
EUR 485 |
Chicken Filamin C, Gamma ELISA Kit |
|
MBS735191-5x96StripWells |
MyBiosource |
5x96-Strip-Wells |
EUR 3020 |
Chicken Filamin C, Gamma ELISA Kit |
|
MBS735191-96StripWells |
MyBiosource |
96-Strip-Wells |
EUR 690 |
Chicken Filamin A, Alpha ELISA Kit |
|
MBS746703-10x96StripWells |
MyBiosource |
10x96-Strip-Wells |
EUR 5685 |
Chicken Filamin A, Alpha ELISA Kit |
|
MBS746703-48StripWells |
MyBiosource |
48-Strip-Wells |
EUR 485 |
Chicken Filamin A, Alpha ELISA Kit |
|
MBS746703-5x96StripWells |
MyBiosource |
5x96-Strip-Wells |
EUR 3020 |
Chicken Filamin A, Alpha ELISA Kit |
|
MBS746703-96StripWells |
MyBiosource |
96-Strip-Wells |
EUR 690 |
) BSA (Standard Grade) |
|
30-AB70 |
Fitzgerald |
1 kg |
EUR 875 |
|
|
|
Description: Standard Grade Bovine Serum Albumin (99% pure) |
BSA (Standard Grade) |
|
MBS537004-1kg |
MyBiosource |
1kg |
EUR 1360 |
BSA (Standard Grade) |
|
MBS537004-5x1kg |
MyBiosource |
5x1kg |
EUR 6025 |
 ELISA Kit) Chicken Filamin B Beta (FLNB) ELISA Kit |
|
abx355875-96tests |
Abbexa |
96 tests |
EUR 990 |
|
|
 ELISA Kit) Chicken Filamin C Gamma (FLNC) ELISA Kit |
|
abx355902-96tests |
Abbexa |
96 tests |
EUR 990 |
|
|
 ELISA Kit) Chicken Filamin A Alpha (FLNA) ELISA Kit |
|
abx356392-96tests |
Abbexa |
96 tests |
EUR 990 |
|
|
 ELISA Kit) Chicken FLNC (Filamin C, Gamma) ELISA Kit |
|
MBS2501401-10x96Tests |
MyBiosource |
10x96Tests |
EUR 4445 |
Chicken FLNC (Filamin C, Gamma) ELISA Kit |
|
MBS2501401-24Tests |
MyBiosource |
24Tests |
EUR 280 |
Chicken FLNC (Filamin C, Gamma) ELISA Kit |
|
MBS2501401-48Tests |
MyBiosource |
48Tests |
EUR 495 |
Chicken FLNC (Filamin C, Gamma) ELISA Kit |
|
MBS2501401-5x96Tests |
MyBiosource |
5x96Tests |
EUR 2480 |
Chicken FLNC (Filamin C, Gamma) ELISA Kit |
|
MBS2501401-96Tests |
MyBiosource |
96Tests |
EUR 585 |
 ELISA Kit) Chicken FLNbeta (Filamin B, Beta) ELISA Kit |
|
MBS2508621-10x96Tests |
MyBiosource |
10x96Tests |
EUR 4445 |
Chicken FLNbeta (Filamin B, Beta) ELISA Kit |
|
MBS2508621-24Tests |
MyBiosource |
24Tests |
EUR 280 |
Chicken FLNbeta (Filamin B, Beta) ELISA Kit |
|
MBS2508621-48Tests |
MyBiosource |
48Tests |
EUR 495 |
Chicken FLNbeta (Filamin B, Beta) ELISA Kit |
|
MBS2508621-5x96Tests |
MyBiosource |
5x96Tests |
EUR 2480 |
Chicken FLNbeta (Filamin B, Beta) ELISA Kit |
|
MBS2508621-96Tests |
MyBiosource |
96Tests |
EUR 585 |
 ELISA Kit) Chicken FLNalpha (Filamin A, Alpha) ELISA Kit |
|
MBS2506863-10x96Tests |
MyBiosource |
10x96Tests |
EUR 4445 |
Chicken FLNalpha (Filamin A, Alpha) ELISA Kit |
|
MBS2506863-24Tests |
MyBiosource |
24Tests |
EUR 280 |
Chicken FLNalpha (Filamin A, Alpha) ELISA Kit |
|
MBS2506863-48Tests |
MyBiosource |
48Tests |
EUR 495 |
Chicken FLNalpha (Filamin A, Alpha) ELISA Kit |
|
MBS2506863-5x96Tests |
MyBiosource |
5x96Tests |
EUR 2480 |
Chicken FLNalpha (Filamin A, Alpha) ELISA Kit |
|
MBS2506863-96Tests |
MyBiosource |
96Tests |
EUR 585 |
 CA15-3, standard grade |
|
MBS664086-1Unit |
MyBiosource |
1Unit |
EUR 1605 |
CA15-3, standard grade |
|
MBS664086-5x1Unit |
MyBiosource |
5x1Unit |
EUR 6885 |
 Chicken Filamin Binding LIM Protein 1 ELISA Kit |
|
MBS074640-INQUIRE |
MyBiosource |
INQUIRE |
Ask for price |
 Tryptase Human Standard Grade |
|
GWB-4CE407 |
GenWay Biotech |
0.1 mg |
Ask for price |
|
|
 Transferrin Receptor Standard Grade |
|
GWB-CDE6A4 |
GenWay Biotech |
1 mg |
Ask for price |
 Florisil@ -200 Mesh Standard Grade |
|
F01454 |
Pfaltz & Bauer |
100G |
EUR 229.1 |
 Standard grade - 610, Dia/Size in cm12.5 |
|
6100-1250-100C |
EWC Diagnostics |
1 unit |
EUR 20.85 |
|
Description: Standard grade - 610, Dia/Size in cm12.5 |
 Standard grade - 961, Dia/Size in cm12.5 |
|
9610-1250-50C |
EWC Diagnostics |
1 unit |
EUR 14.2 |
|
Description: Standard grade - 961, Dia/Size in cm12.5 |
 Standard grade - 961, Dia/Size in 46x57 |
|
9610-4657-100S |
EWC Diagnostics |
1 unit |
EUR 424.61 |
|
Description: Standard grade - 961, Dia/Size in 46x57 |
 Standard grade - 131, Dia/Size in cm 4.7 |
|
1310-0470-100C |
EWC Diagnostics |
1 unit |
EUR 82.59 |
|
Description: Standard grade - 131, Dia/Size in cm 4.7 |
 Standard grade - 161, Dia/Size in cm 4.7 |
|
1610-0470-100C |
EWC Diagnostics |
1 unit |
EUR 88.63 |
|
Description: Standard grade - 161, Dia/Size in cm 4.7 |
 Standard grade - 601, Dia/Size in cm 11 |
|
6010-1100-100C |
EWC Diagnostics |
1 unit |
EUR 11.84 |
|
Description: Standard grade - 601, Dia/Size in cm 11 |
 Standard grade - 601, Dia/Size in cm 12. |
|
6010-1250-100C |
EWC Diagnostics |
1 unit |
EUR 12.79 |
|
Description: Standard grade - 601, Dia/Size in cm 12. |
 Standard grade - 601, Dia/Size in cm 15 |
|
6010-1500-100C |
EWC Diagnostics |
1 unit |
EUR 16.27 |
|
Description: Standard grade - 601, Dia/Size in cm 15 |
 Standard grade - 601, Dia/Size in cm 46 |
|
6018-4657-100S |
EWC Diagnostics |
1 unit |
EUR 197.06 |
|
Description: Standard grade - 601, Dia/Size in cm 46 |
 Standard grade - 610, Dia/Size in cm 15 |
|
6100-1500-100C |
EWC Diagnostics |
1 unit |
EUR 32.68 |
|
Description: Standard grade - 610, Dia/Size in cm 15 |
 Standard grade - 613, Dia/Size in cm 4.7 |
|
6130-0470-100C |
EWC Diagnostics |
1 unit |
EUR 7.89 |
|
Description: Standard grade - 613, Dia/Size in cm 4.7 |
 Standard grade - 613, Dia/Size in cm 7.5 |
|
6130-0750-100C |
EWC Diagnostics |
1 unit |
EUR 8.69 |
|
Description: Standard grade - 613, Dia/Size in cm 7.5 |
 Standard grade - 613, Dia/Size in cm 11 |
|
6130-1100-100C |
EWC Diagnostics |
1 unit |
EUR 11.53 |
|
Description: Standard grade - 613, Dia/Size in cm 11 |
 Standard grade - 613, Dia/Size in cm 50 |
|
6130-5050-100S |
EWC Diagnostics |
1 unit |
EUR 169.94 |
|
Description: Standard grade - 613, Dia/Size in cm 50 |
 Standard grade - 615, Dia/Size in cm 12. |
|
6150-1250-100C |
EWC Diagnostics |
1 unit |
EUR 18.27 |
|
Description: Standard grade - 615, Dia/Size in cm 12. |
 Standard grade - 615, Dia/Size in cm 18. |
|
6150-1850-100C |
EWC Diagnostics |
1 unit |
EUR 33.29 |
|
Description: Standard grade - 615, Dia/Size in cm 18. |
 Standard grade - 615, Dia/Size in cm 46 |
|
6150-4657-100S |
EWC Diagnostics |
1 unit |
EUR 197.98 |
|
Description: Standard grade - 615, Dia/Size in cm 46 |
 Standard Grade -631 , Dia/size in cm 7 |
|
6310-0700-100C |
EWC Diagnostics |
1 unit |
EUR 10.42 |
|
Description: Standard Grade -631 , Dia/size in cm 7 |
 Standard Grade -631 , Dia/size in cm 9 |
|
6310-0900-100C |
EWC Diagnostics |
1 unit |
EUR 11.84 |
|
Description: Standard Grade -631 , Dia/size in cm 9 |
 Standard grade - 631, Dia/Size in cm 12. |
|
6310-1250-100C |
EWC Diagnostics |
1 unit |
EUR 17.84 |
|
Description: Standard grade - 631, Dia/Size in cm 12. |
 Standard Grade =642 , Dia/size in 7 cm |
|
6420-0700-100C |
EWC Diagnostics |
1 unit |
EUR 13.41 |
|
Description: Standard Grade =642 , Dia/size in 7 cm |
 Standard Grade =642 , Dia/size in 9 cm |
|
6420-0900-100C |
EWC Diagnostics |
1 unit |
EUR 13.41 |
|
Description: Standard Grade =642 , Dia/size in 9 cm |
 Standard Grade =642 , Dia/size in 11 cm |
|
6420-1100-100C |
EWC Diagnostics |
1 unit |
EUR 14.86 |
|
Description: Standard Grade =642 , Dia/size in 11 cm |
 Standard grade - 642, Dia/Size in cm 12. |
|
6420-1250-100C |
EWC Diagnostics |
1 unit |
EUR 16.27 |
|
Description: Standard grade - 642, Dia/Size in cm 12. |
 Standard grade - 642, Dia/Size in cm 46 |
|
6428-4657-100S |
EWC Diagnostics |
1 unit |
EUR 954.89 |
|
Description: Standard grade - 642, Dia/Size in cm 46 |
 Standard grade - 961, Dia/Size in cm 9 |
|
9610-0900-100C |
EWC Diagnostics |
1 unit |
EUR 22.26 |
|
Description: Standard grade - 961, Dia/Size in cm 9 |
 Standard grade - 961, Dia/Size in cm 11 |
|
9610-1100-100C |
EWC Diagnostics |
1 unit |
EUR 26.7 |
|
Description: Standard grade - 961, Dia/Size in cm 11 |
 Standard grade - 961, Dia/Size in cm 12. |
|
9610-1250-100C |
EWC Diagnostics |
1 unit |
EUR 32.68 |
|
Description: Standard grade - 961, Dia/Size in cm 12. |
 Standard grade - 961, Dia/Size in cm 15 |
|
9610-1500-100C |
EWC Diagnostics |
1 unit |
EUR 44.54 |
|
Description: Standard grade - 961, Dia/Size in cm 15 |
 Proteinase K, Recombinant, Standard Grade |
|
9250-100 |
Biovision |
each |
EUR 124.8 |
Proteinase K, Recombinant, Standard Grade |
|
9250-10G |
Biovision |
each |
EUR 4627.2 |
Proteinase K, Recombinant, Standard Grade |
|
9250-1G |
Biovision |
each |
EUR 548.4 |
Proteinase K, Recombinant, Standard Grade |
|
9250-500 |
Biovision |
each |
EUR 314.4 |
Proteinase K, Recombinant, Standard Grade |
|
9251-100 |
Biovision |
each |
EUR 1266 |
Proteinase K, Recombinant, Standard Grade |
|
9251-25 |
Biovision |
each |
EUR 352.8 |
Proteinase K, Recombinant, Standard Grade |
|
9251-5 |
Biovision |
each |
EUR 144 |
 Standard Grade-610, Dia/size in 7 cm |
|
6100-0700-100C |
EWC Diagnostics |
1 unit |
EUR 13.41 |
|
Description: Standard Grade-610, Dia/size in 7 cm |
 Chicken Immunoglobulin Reference Standard |
|
GWB-DDLDDL |
GenWay Biotech |
1.0 ml |
Ask for price |
 Chicken Ovotransferrin Reference Standard |
|
MBS563337-INQUIRE |
MyBiosource |
INQUIRE |
Ask for price |
 Standard grade native Human HE4 protein |
|
MBS5308336-1000pM |
MyBiosource |
1000pM |
EUR 370 |
Standard grade native Human HE4 protein |
|
MBS5308336-5x1000pM |
MyBiosource |
5x1000pM |
EUR 1520 |
 Standard grade - 601, Dia/Size in cm 7 R |
|
6010-0700-100C |
EWC Diagnostics |
1 unit |
EUR 15.8 |
|
Description: Standard grade - 601, Dia/Size in cm 7 R |
 Standard grade - 601, Dia/Size in cm 9 R |
|
6010-0900-100C |
EWC Diagnostics |
1 unit |
EUR 10.42 |
|
Description: Standard grade - 601, Dia/Size in cm 9 R |
 Standard grade - 613, Dia/Size in cm 7 R |
|
6130-0700-100C |
EWC Diagnostics |
1 unit |
EUR 8.69 |
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Description: Standard grade - 613, Dia/Size in cm 7 R |
To systematically interrogate metabolic vulnerabilities in PDA, we employed parallel CRISPR-Cas9 screens utilizing in vivo and in vitro techniques. This work revealed placing overlap of in vivo metabolic dependencies with these in vitro. Furthermore, we recognized that intercellular nutrient sharing can masks dependencies in pooled screens, highlighting a limitation of this strategy to review tumor metabolism.
